An affinity-based fluorescence polarization assay for protein tyrosine phosphatases
نویسندگان
چکیده
منابع مشابه
Continuous assay of protein tyrosine phosphatases based on fluorescence resonance energy transfer.
An assay method that continuously measures the protein tyrosine phosphatase (PTP)-catalyzed dephosphorylation reaction based on fluorescence resonance energy transfer (FRET) was developed as an improvement of our previously reported discontinuous version [M. Nishikata, K. Suzuki, Y. Yoshimura, Y. Deyama, A. Matsumoto, Biochem. J. 343 (1999) 385-391]. The assay uses oligopeptide substrates that ...
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ژورنال
عنوان ژورنال: Methods
سال: 2007
ISSN: 1046-2023
DOI: 10.1016/j.ymeth.2007.02.008